| --version |
None |
show program's version number and exit |
| --iter |
int |
Iteration number within path. Default = start a new iteration |
| --simthr |
float |
Similarity is smaller for better 'quality' particles. Specify the highest value to include from e2spt_hist.py. Default -0.1 |
| --simthr2 |
float |
Simlarity score for the best matching final alignment. Scaling may be different due to resolution limit. Default 0 |
| --replace |
str |
Replace the input subtomograms used for alignment with the specified file (used when the aligned particles were masked or filtered) |
| --wedgesigma |
float |
Threshold for identifying missing data in Fourier space in terms of standard deviation of each Fourier shell. Default 3.0 |
| --minalt |
float |
Minimum alignment altitude to include. Default=0 |
| --maxalt |
float |
Maximum alignment altitude to include. Deafult=180 |
| --maxtilt |
float |
Explicitly zeroes data beyond specified tilt angle. Assumes tilt axis exactly on Y and zero tilt in X-Y plane. Default 90 (no limit). |
| --maxres |
float |
Lowpass filter applied to particles prior to alignment/averaging, resolution in A. Default disabled |
| --listfile |
str |
Specify a filename containing a list of integer particle numbers to include in the average, one per line, first is 0. Additional exclusions may apply. |
| --shrinkcompare |
int |
Shrink factor for classification only (for speed) |
| --sym |
str |
Symmetry of the input. Must be aligned in standard orientation to work properly. The structure will be expanded from this symmetry to c1. |
| --applysym |
str |
Symmetry to apply to the structure after classification. |
| --path |
str |
Path to a folder containing current results (default = highest spt_XX) |
| --parallel |
str |
parallel mode. Not all functions are implemented yet.. |
| --threads |
int |
Number of alignment threads to run in parallel on a single computer. This is the only parallelism supported by e2spt_align at present. |
| --maskclass |
str |
Mask each reference before classification |
| --mask |
str |
Mask applied to final averages |
| --verbose, -v |
int |
verbose level [0-9], higher number means higher level of verboseness |
| --noali |
None |
Skip translational alignment. |
| --symcopy |
None |
Copy each particle for each asymetrical unit. need a maskclass to focus on one unit. do not work with applysym |
| --nolstout |
None |
Skip writting lst output. |
| --sample |
int |
use only N samples. |
| --randnclass |
int |
split into N random classes. ignore refs |
| --ppid |
int |
Set the PID of the parent process, used for cross platform PPID |