| --version |
None |
show program's version number and exit |
| --path |
str |
Path to an existing spt_XX folder with the alignment results to use, defualt = highest spt_XX |
| --iter |
int |
Iteration number within path, default = last iteration |
| --ncls |
int |
Number of classes to generate |
| --nbasis |
int |
Number of basis vectors for the MSA phase, default=4 |
| --layers |
int |
number of 1 pixel layers about the center to use for the projection in each direction (size in reduced image if --shrink used), ie 0->1, 1->3, 2->5. Default=2 |
| --sym |
str |
Symmetry of the input. Must be aligned in standard orientation to work properly. |
| --shrink |
int |
shrink the particles before processing |
| --mask |
str |
A 3D mask file or a single mask processor specification to apply prior to local projection generation |
| --threads |
int |
Number of alignment threads to run in parallel on a single computer. This is the only parallelism supported by e2spt_align at present. |
| --hp |
float |
Apply a high-pass filter at the specified resolution when generating projections. Specify as resolution in A, eg - 100 |
| --lp |
float |
Apply a low-pass filter at the specified resolution when generating projections. Specify the resolution in A, eg - 25 |
| --saveali |
None |
In addition to the unaligned sets/ for each class, generate aligned particle stacks per class |
| --verbose, -v |
int |
verbose level [0-9], higher number means higher level of verboseness |
| --ppid |
int |
Set the PID of the parent process, used for cross platform PPID |