--version |
None |
show program's version number and exit |
--ptcls |
str |
Particle input. required |
--path |
str |
Path for the refinement |
--nref |
int |
Number of classes. Without --loadali3d, it duplicate the first ref N times with phase randomization at 2 x maxres. With --loadali3d, the particles are classified to N random classes at the begining. |
--maskalign |
str |
Mask file applied to 3D alignment reference in each iteration. Not applied to the average, which will follow normal masking routine. |
--maskref |
str |
Mask applied to the references prior to classification |
--maxres |
float |
Maximum resolution (the smaller number) to consider in alignment (in A, not 1/A). Default is 20A |
--minres |
float |
Minimum resolution (the larger number) to consider in alignment (in A, not 1/A) |
--niter |
int |
number of iterations. default is 5. |
--loadali3d |
None |
load previous 3d alignment from --ptcls input. |
--skipali |
None |
Skip alignment entirely when --loadali3d is provided. Otherwise a local orientation search will still be performed. |
--parallel |
str |
parallel options |
--sym |
str |
symmetry to apply to the average structure |
--breaksym |
str |
Break specified symmetry. Only used when --loadali3d is on. |
--setsf |
str |
set structure factor from text file |
--maxshift |
int |
maximum shift for local alignment. default box size/6 |
--maxang |
int |
maximum angle difference for local alignment. |