| --version |
None |
show program's version number and exit |
| --ptcls |
str |
Particle input. required |
| --path |
str |
Path for the refinement |
| --nref |
int |
Number of classes. Without --loadali3d, it duplicate the first ref N times with phase randomization at 2 x maxres. With --loadali3d, the particles are classified to N random classes at the begining. |
| --maskalign |
str |
Mask file applied to 3D alignment reference in each iteration. Not applied to the average, which will follow normal masking routine. |
| --maskref |
str |
Mask applied to the references prior to classification |
| --maxres |
float |
Maximum resolution (the smaller number) to consider in alignment (in A, not 1/A). Default is 20A |
| --minres |
float |
Minimum resolution (the larger number) to consider in alignment (in A, not 1/A) |
| --niter |
int |
number of iterations. default is 5. |
| --loadali3d |
None |
load previous 3d alignment from --ptcls input. |
| --skipali |
None |
Skip alignment entirely when --loadali3d is provided. Otherwise a local orientation search will still be performed. |
| --parallel |
str |
parallel options |
| --sym |
str |
symmetry to apply to the average structure |
| --breaksym |
str |
Break specified symmetry. Only used when --loadali3d is on. |
| --setsf |
str |
set structure factor from text file |
| --maxshift |
int |
maximum shift for local alignment. default box size/6 |
| --maxang |
int |
maximum angle difference for local alignment. |