usage: prog [options] Tiltvalidation using Richard Henderson's technique. To use a stack of untilted and tiltimages whose set relationship is one-to-one is required along with a volume to validate. This can be generated using e2RCTboxer.py. After running this program two bits of data are products. A contour plot similar to Figure 5 in the Henderson paper(see below), and a list of titlangles and tiltaxes between particle paris, which can be used to makes plot similar to Figure 6 in Hendersons paper. The contour plot is stored as contour.hdf and the tiltpairs data is stored as bdb:perparticletilts. For more information see: Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. Rosenthal PB, Henderson R. J Mol Biol. 2003 Oct 31;333(4):721-45
Option | Type | Description |
---|---|---|
--version | None | show program's version number and exit |
--untiltdata | str | Stack of untilted images |
--tiltdata | str | Stack of tilted images |
--volume | str | 3D volume to validate |
--maxtiltangle | float | Maximum tiltangle permitted when finding tilt distances |
--quaternion | None | Use Quaterions for tilt distance computation |
--sym | str | The recon symmetry |
--docontourplot | None | Compute a contour plot |
--tiltrange | int | The angular tiltrange to search |
--align | str | The name of a aligner to be used in comparing the aligned images |
--cmp | str | The name of a 'cmp' to be used in comparing the aligned images |
--delta | float | The angular step size for alingment |
--shrink | int | Optionally shrink the input particles by an integer amount prior to computing similarity scores. For speed purposes. Defulat = 0, no shrinking |
--simcmp | str | The name of a 'cmp' to be used in comparing the aligned images (default=ccc) |
--simalign | str | The name of an 'aligner' to use prior to comparing the images (default=rotate_translate) |
--simaligncmp | str | Name of the aligner along with its construction arguments (default=ccc) |
--simralign | str | The name and parameters of the second stage aligner which refines the results of the first alignment |
--simraligncmp | str | The name and parameters of the comparitor used by the second stage aligner. (default=dot). |
--parallel | str | Parallelism string |
--verbose | int | verbose level [0-9], higher number means higher level of verboseness |
--path | str | The folder the results are placed |
--radcut | float | For use in the GUI, truncate the polar plot after R. -1 = no truncation |
--gui | None | Start the GUI for viewing the tiltvalidate plots |
--planethres | float | Maximum out of plane threshold for the tiltaxis. 0 = perfectly in plane, 1 = normal to plane |
--datalabelscolor | str | Set the color of the data labels. Any vaild matplotlib color is ok |
--datalabels | None | Add data labels to the plot |
--colorzaxis | None | Color scatter dots by Z axis |
--eulerfile | str | Euler angles file, to create tiltdistance from pre-aligned particles. Format is: imgnum, name, az, alt, phi |
--ppid | int | Set the PID of the parent process, used for cross platform PPID |
For more information go to emanwiki/EMAN2/Programs/e2tiltvalidate.