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NCMI Workshops: Cryo EM Modeling

Workshop on Single Particle Reconstruction, Structural Variability and Modeling: Oct 30 - Nov 2, 2015

Presentation notes from the Workshop (some of these have not been uploaded yet)

Video of selected sessions Our primary video recording mechanism failed to record audio, so we unfortunately have only the EMAN and Modeling tutorials recorded

Tutorial PDFs

Tutorial Data You will also need all of the demonstration data, which we will use when going through the tutorials:

Completed Tutorial Projects (large files)

Software used at the workshop:

  • EMAN/SPARX 2.12 (or snapshot version released after Oct. 19, 2015) - (day 1 - 2) Required. Do not use an older version.

  • Relion 1.4 (optional, day 2-3) - The Relion session will largely be a demo. See comments below.

  • Chimera Daily Build Oct. 17 or later - For PDB viewing and Segger tutorial (day 4, specific version required)

  • Warning: we are aware of an issue with Gorgon on Ubuntu. Will remove this warning when resolved. Other Linux variants should be fine, now.
  • Gorgon - Gorgon Tutorial (day 4) Please install the appropriate binary AND

  • Phenix - (end of day 4) real-space refinement of models

  • LKH Solver - (day 4 - pathwalker), required only for the pathwalker tutorial, see below

  • OpenMPI and PyDusa (day 2-3) SPARX tutorial may also largely be a demo, see below

Modeling of Cryo-EM map Workshop: Jan 14 - Jan 17, 2010


Workshop on Single Particle Reconstruction, Map Interpretation and Visualization: Dec 10 - Dec 13, 2008


Workshop on Single Particle Reconstruction and Visualization: Mar. 14, 2007


Visualization of Large Biomolecular Complexes: Sep. 11 - Sep. 13, 2005


Workshop on Structural and Computational Proteomics of Biological Complexes: May. 16 - May. 17, 2005


Single Particle Reconstructions and Visualization EMAN Tutorial: Mar. 16 - Mar. 19, 2005


Visualization of Biological Complexes: Oct. 11 - Oct. 12, 2003


Workshop on Structural Proteomics of Biological Complexes: Apr. 7 - Apr. 8, 2003


  • Chiu_Visualization_workshop.ppt - Workshop Goals - Wah Chiu

  • Drubin.ppt - Why Studying Large Protein Complexes is Crucial at this Time - David Drubin

  • Jack Greenblatt.ppt - Protein Complexes in Transcription - Jack Greenblatt

  • - Chromosome Segregation and Kinetochore Structure in Yeast - Peter K. Sorger

  • - Protein Complexes in Cell Division in Eggs from Xenopus Laevis - Rebecca Heald

  • Yates.ppt - Analysis of Protein Complexes by Mass Spectrometry - John Yates

  • Boone.ppt - Synthetic Genetic Array Analysis: Large-Scale Mapping of Genetic Interactions Networks in Yeast - Charlie Boone

  • - Mining Relevance from the Mycobacterium Tuberculosis Genome and Structural Database - William Jacobs

  • Gerstein.ppt - Computational Proteomics: Prediction of Protein Interactions and Function on a Genome-Scale - Mark Gerstein

  • Sali.ppt - Modeling the Structures of Molecular Assemblies by Satisfaction of Spatial Restraints - Andrej Sali

  • Downing.ppt - Electron Crystallography: Current State and Prospects - Ken Downing

  • Milligan.ppt - The Combined Use of Cryo-Electron Microscopy and X-Ray Crystallography to Understand the Structure and Mechanisms of Molecular Machines - Ron Milligan

  • Almo.ppt - Structural Proteomics: Structural Dissection of Protein Machines - Steve Almo

  • - Structural Proteomics of Infectious Agents - David Stuart

  • Kurosky.ppt - Proteomics in Airway Inflammation - Alex Kurosky

  • Buchanan.ppt - New Approaches for High-Throughput Identification and Characterization of Protein Complexes - Michelle Buchanan

  • Davis.ppt - Yeast Resource Center - Trisha Davis

  • Makowski.ppt - Realistic Goals and Milestones; Resources Needed and Funding Mechanisms - Lee Makowski

National Center for Macromolecular Imaging Workshop on Single Particle Reconstructions and Visualization (& EMAN Tutorial): Dec. 11 - Dec. 15, 2002