== NCMI Workshops: Cryo EM specimen preparation == ''' Sample preparation Videos: ''' ''Click the links below to watch the videos'' *[[http://blake.bcm.edu/dl/NCMI_training/Cryo_Transfer.mov|Cryo transfer]] *[[http://blake.bcm.edu/dl/NCMI_training/Plasma_Cleaning.mov|Plasma cleaning]] *[[http://blake.bcm.edu/dl/NCMI_training/Tutorial_Cp3.mov|Freezing using Gatan Cp3]] ---- ''' [[http://ncmi.bcm.edu/ncmi/events/workshops/workshops_83|CryoEM Training Workshop: June 8, 2007]] ''' '''Presentations''' * [[http://blake.bcm.edu/dl/NCMI_training/Cryo_Transfer.mov|Cryo transfer]] - Cruciano, Angela; Rees, Ian * [[attachment:Chiu handout 1 07-06-08.ppt||&do=get]] - Day 1 Lecture- Overview of Cryo EM - Wah Chiu * [[attachment:MDS Alignment.ppt||&do=get]] - MDS alignment - Cruciano, Angela ---- ''' [[http://ncmi.bcm.edu/ncmi/events/workshops/workshops_64|CryoEM Workshop: Oct. 17, 2006]] ''' '''Presentations''' * [[attachment:2006Oct18CryoEMCourseCCDs.ppt||&do=get]] - Introduction To Using CCD Cameras In cryo-EM - Booth, Christopher * [[attachment:CHIU Handout CryoEM Rice 06-10-18.ppt||&do=get]] - CryoEM Lecture for Biophysics Course and Workshop on Oct 18 2006 - Wah Chiu * [[attachment:Cryo TEM from specimen prep to the microscope_Gatan Presentation_LAM1.pdf]] - Cryo TEM from specimen prep to the microscope - Linda Melanson * [[attachment:CryoEM_Imaging_NCMI_20061018.pdf||&do=get]] - Imaging in Cryo-electron microscopy - Brink, Jaap * [[attachment:Frederik.pdf]] - CryoEM of supsension - Professor Peter Frederik ---- ''' [[http://ncmi.bcm.edu/ncmi/events/workshops/workshops_53|CryoEM Tutorial: Jul. 25 - Jul. 29, 2005]] ''' '''Presentations''' * [[attachment:cryoEM workshop 05_07_26.ppt||&do=get]] - Basics of CryoEM - Wah Chiu * [[attachment:cryo_EM-workshop_7-25-05.ppt||&do=get]] - EM grid preparation - Zongli Li * [[attachment:cryoEM2005_Serysheva.ppt||&do=get]] - Vitrification of biological specimen - Serysheva, Irina * [[attachment:2005July26_DetectingElectronsLecture.ppt||&do=get]] - Detecting Electrons: CCD vs Film - Christopher Booth ---- ''' [[http://ncmi.bcm.edu/ncmi/events/workshops/workshops_8|Tutorial of TEM for Biological Cryo-Specimens: Oct. 11, 2004]] ''' '''Presentations''' * [[attachment:EMtutorial.pdf||&do=get]] - EM Tutorial (2004) - Wah Chiu * [[attachment:gridClean_Li.ppt ||&do=get]] - Grid Cleaning - Zongli Li ---- ''' [[http://ncmi.bcm.edu/ncmi/events/workshops/workshops_43|Tutorial on CryoEM: Oct. 18, 2003]] ''' '''Presentations''' * [[attachment:EM Tutorial_03_10_18.ppt||&do=get]] - Basics of electron microscope - Wah Chiu * [[attachment:EMPresentation_Glen_1018-2003.ppt ||&do=get]] - Grid for TEM - Glen Decker * [[attachment:ISvitrification2003.ppt ||&do=get]] - Theory on Cryo-EM Preparation - Irina Serysheva ----