Type

Extension

Read

Write

3D

stack

Comments

MRC

mrc

Y

Y

Y

N

Spider

spi

Y

Y

Y

Y

To read the overall image header in a stacked spider file, use image_index = -1.

Imagic

img/hed

Y

Y

Y

Y

seperate header and data file, cannot store multiple 3D images in one file

HDF5

hdf

Y

Y

Y

Y

can write different size image into same stack

DM3

dm3

Y

N

N

N

TIFF

tiff/tif

Y

Y

N

N

8bit or 16bit per pixel

PGM

pgm

Y

Y

N

N

8 bits per pixel

LST

lst

Y

Y

Y

Y

ASCII file contains a list of image file names

PIF

pif

Y

Y

Y

Y

images in PIF stack are homogenous

VTK

vtk

Y

Y

Y

N

PNG

png

Y

Y

N

N

lossless data compression, 8 bit or 16 bit per pixel

SAL

hdr/img

Y

N

N

N

seperate header and data file

ICOS

icos

Y

Y

Y

N

EMIM

emim

Y

N

Y

Y

images in stack are homogenous

Gatan2

dm2

Y

N

N

N

Amira

N

Y

Y

N

XPLOR

xplor

Y

Y

Y

N

8 bytes integer, 12.5E float format

EM

em

Y

Y

Y

N

V4L

v4l

Y

N

N

N

Acquires images from the V4L2 interface in real-time(video4linux).

JPEG

jpg/jpeg

N

Y

N

lossy data compression

FITS

fts

Y

N

Y

N

common file format in astronomy

LSTFAST

lsx/lst

Y

Y

Y

Y

ASCII file contains a list of image file names