e2ctf

CTF processing in 3 stages

To get familiar with how e2ctf.py works, try separating the way you using e2ctf.py into three stages:

Auto fitting

e2ctf.py  1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit

Fine tuning

(save any changes you make with the Save Parms button)

e2ctf.py  1.img --gui

Write output

e2ctf.py 1.img --phaseflip --wiener

The output will be in the particles directory. Also, you can supply more than one image as input to e2ctf.py.

Processing multiple images simultaneously

e2ctf takes multiple images as input, so you can use commands like this:

e2ctf.py *.img --phaseflip --wiener

Combining stages into a single command

You can combine more than one of the e2ctf processing stages into a single command. For instance you can run automated fitting and force the opening of the GUI in a with a single command like this:

e2ctf.py  1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui