e2ctf

Contents

  1. e2ctf
    1. CTF processing in 3 stages
      1. Auto fitting
      2. Fine tuning
      3. Write output
    2. Processing multiple images simultaneously
    3. Combining stages into a single command

ctf.png

CTF processing in 3 stages

To get familiar with how e2ctf.py works, try separating the way you using e2ctf.py into three stages:

Auto fitting

e2ctf.py  1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit

This runs auto CTF parameter determination, storing the result in a local database.

Fine tuning

e2ctf.py  1.img --gui

Now that you have determined the parameters with the first command, have a look at the results in the interface. Tweak if necessary. Save any changes you make with the Save Parms button

Write output

e2ctf.py 1.img --phaseflip --wiener

Finally generate output. The output will be in the particles directory. Also, you can supply more than one image as input to e2ctf.py.

Processing multiple images simultaneously

e2ctf takes multiple images as input, so you can use commands like this: 

e2ctf.py  *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit
e2ctf.py  *.img --gui
e2ctf.py *.img --phaseflip --wiener

Combining stages into a single command

You can combine more than one of the e2ctf processing stages into a single command. For instance you can run automated fitting and open the GUI with a single command like this: 

e2ctf.py  1.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui
e2ctf.py  *.img --voltage=200 --apix=2 --cs=3.2 --auto_fit --gui