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e2refinetorelion3d Tutorial

Basic Information

This tutorial is designed with 2 assumptions in mind:


The first thing to do, assuming you've installed Relion and run an EMAN2 reconstruction is to open up the Project Manager:

EMAN2 Project Manager

Once you open up the project manager, select the plus (+) next to the 3D refinement tab and you will see several new options appear. The option we want to select is the "Relion 3D Refinement" option.


This opens up three tabs in the viewing window: GUI, Command, and Help. 'GUI' contains the options for, 'Command' is the command that will be executed when the user launches the program, and 'Help' is the help text associated with Along the right side, you will see 7 option buttons with various shapes. (More information about the project manager can be found here) The Three buttons that are directly impacted by this program are: Wiki Button *The Wiki Button Wizard Button *The Wizard Button Expert Button *The Expert Button

In the 'GUI' window, there are several sections of options. The first set options you will see are the I/O options.

I/O Options

set_name: This is the name of the particle set that will be used as input to Relion. By clicking 'Browse', you will open the EMAN2 browser and be able select the set by browsing to it.
refmap : This is the 3D map that will be used as a reference map by Relion. It should have the same dimensions as your particles. Typically you should use an EMAN2 completed reconstruction as input.
randomizemodel: This is an option that can be used to