NCMI Workshops: Presentations and Videos Archive

Workshop on Single Particle Reconstruction, Structural Variability and Modeling: Oct 30 - Nov 2, 2015

Presentations

• wah-introduction.pdf - Introduction to the Workshop and NCMI

• ludtke-overview.pdf - Workshop Overview - Steve Ludtke

• mcmullanintro.pdf - Electron Microscopes & Imaging Artifacts - Greg McMullan

• penczek-alignment.pdf - 2D Alignment Theory - Pawel Penczek

• ludtke-minitalks.pdf - CTF, Refinement, other topics - Steve Ludtke

• baldwin-orientation.pdf - Orientation and Symmetry - Phil Baldwin

• scheres-heterogeneity.pdf - Methods for Heterogeneity Analysis - Sjors Scheres

• mcmullanDetector.pdf - Direct Detector Technology - Greg McMullan

• pintilie.pdf - Chimera & Segger - Greg Pintilie

• baker-model-building.pdf - Model Building - Matt Baker

• lawson-challenges.pdf - EMDB Challenges - Cathy Lawson

Tutorials

• EMAN2-12_Tutorial.pdf

• EMAN2-12_Tutorial2_heterogeneity.pdf

  • Please note that e2refine_split has been improved significantly since 2.12. Suggest an update if using this method.

• Segger Tutorials

• PathwalkerGorgonTutorial.pdf

• ModelOptimizationTutorial.pdf

• ModelValidationTutorial.pdf

Cryo-EM Workshop at the National University of Singapore: July 9 - July 13, 2012

Presentations

• emviz.pdf - Map Visualization with Chimera - Tom Goddard

• CHIU CryoEM lecture NUS workshop 2012.pdf - CryoEM Basics - Wah Chiu

• Model_bias.pdf - Model Bias- Mini Lecture Quantitative - Steve Ludtke

• Particle_Eval.pdf - Particle Analysis (mini-lecture) - Steve Ludtke

• EMAN2_Intro.pdf - EMAN2 Introduction - Steve Ludtke

• Single_Particle_Intro.pdf - Introduction to Single Particle Analysis - Steve Ludtke

• Lu Gan NUS cryo-ET lecture.pdf - Cryo-ET Basics - Lu Gan

• Slides_for_workshop3_handout_red.pdf - Single Particle Tomography slides/handout - Michael Schmid et al

• Flagella01-07-10-2012_red.pdf - Trypanosome Flagella - Schmid, M, Koyfman AY, Gheiratmand L, Fu CJ, Khant HA, Huang D, He CY, Chiu W

• CHIU CryoEM Modeling NUS-reduced.pdf - CHIU Modeling Lecture - Wah Chiu

Modeling of Cryo-EM map Workshop: Jan 14 - Jan 17, 2010

Presentations

• modeling2010_tao.pdf - Geometric Algorithms for De Novo Modeling - Abeysinghe, Sasakthi; Schuhl, Steven; Abrams, Austin; Ju, Tao; Baker, Matthew; Chiu, Wah

• PDB_Items_for_EM.ppt - PDB Items for EM (EMDB Schema) - Abeysinghe, Sasakthi; Schuhl, Steven; Abrams, Austin; Ju, Tao; Baker, Matthew; Chiu, Wah

• HoustonWorkshopJan2010-v4.ppt - EM Maps and Models in EMDB/PDB - Abeysinghe, Sasakthi; Schuhl, Steven; Abrams, Austin; Ju, Tao; Baker, Matthew; Chiu, Wah

• xray-validation-for-em.ppt - Applied common sense: The why, what and how of validation - Kleywegt, Gerard J.

• Baker-workshop2010.pdf - De Novo Modeling with Cryo-EM Density Maps - Baker, Matthew

• 2010-01-16 NCMI Baylor Workshop 90min Sali.pdf - Integrative structure determination at pseudo-atomic resolution - Andrej Sali

• 2010 Jan Mesoscale CryoEM Workshop.pdf - MESOSCALE MODELING OF MACROMOLECULAR MACHINES - Michael Levitt

• EMdepoInstructions2010.doc - EM Depo Instructions - Lawson, Cathy

• Schulten1.pdf - Molecular Modeling Flexible Fitting 1: Introduction to Molecular Dynamics - Schulten, Klaus

• Schulten2.pdf - Molecular Modeling Flexible Fitting 2: Introduction to Method - Schulten, Klaus

• Schulten3.pdf - Molecular Modeling Flexible Fitting 3: Application to Ribosome - Schulten, Klaus

• EMD5127-3iyd.ppt - Deposition example EMD-5127-PDB-3iyd - Lawson, Cathy

• EMdepoTutorial2010.doc - EM Deposition Tutorial - Lawson, Cathy

• talk-houston_jan10-schroeder.pdf - Structure Refinement at Low Resolution - Schroeder, Gunnar

Structural & Computational Biomedical Informatics and Cryo-EM (UK): June 8 - June 12, 2009

Presentations

• workflow_tutorial_final.pdf - EMAN2 Workflow Tutorial (Revised) - Steve Ludtke

• Workshop_1.pdf - Introduction to Single Particle Reconsruction and EMAN2 - lecture notes - Steve Ludtke

Workshop on Single Particle Reconstruction, Map Interpretation and Visualization: Dec 10 - Dec 13, 2008

Presentations

• Ludtke_1.pdf - Introduction to Single Particle Reconsruction and EMAN2 - Steve Ludtke

• workflow_tutorial.pdf - EMAN2 Workflow Tutorial - David Woolford

• Python_1.pdf - Introduction to Python - Steve Ludtke

• berman-NCMI-dec08.ppt - What the Protein Data Bank teaches us about structural biology - Berman, Helen M.

• Wrks_EMAN2_08.pdf - Introduction - Penczek, Pawel

• 2008-EMAN-Wen-Jiang.pdf - Near-atomic resolution 3-D reconstruction of viruses - Jiang, Wen

• 2008-12-11 NCMI Baylor Workshop 60min.pdf - Integrating Diverse Data for Structure Determination of Macromolecular Assemblies - Andrej Sali

EMAN/Single Particle Tutorial: June 11, 2008

Presentations

• Talk1.pdf - Introduction to Single Particle Reconstruction in EMAN1 - Steve Ludtke

• Talk2-ctf.pdf - Introduction to Fourier Transforms and CTF Correction - Steve Ludtke

• Demo1.pdf - Demo session containing walkthrough for EMAN1 DVD - Steve Ludtke

• fits.pdf - FFT's Part 1 (more detailed lecture) - Steve Ludtke

• ffts2.pdf - FFT's Part 2 (more detailed lecture) - Steve Ludtke