Diff for "Ws2011/Spt"

Differences between revisions 1 and 6 (spanning 5 versions)

Single Particle Tomography in EMAN2

Wed PM Practical

This is a relatively new feature in EMAN2. This session will cover the beta version of a small fraction of the Single Particle Tomography (SPT) capabilities that will eventually be available.

The session will cover: 1) Sub-volume extraction from tomograms using e2tomoboxer.py 2) Preparation of extracted particles for alignment 3) Reference based alignment and averaging

The software is provided as part of EMAN2 (already installed).

spt_demo_2011.tgz

We have prepared some sample data. The tomogram in the link below comes from a tilt series of epsilon15 virion particles recorded using Zernike phase-plate technology:

e15phaseplate.rec

The following tomogram also comes from a tilt series of epsilon15 virion particles but was recorded under conventional cryoEM imaging conditions:

e15normal.rec

-  ⇤ ← Revision 1 as of 2011-02-04 18:31:10 → 
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  Editor: SteveLudtke
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+   ← Revision 6 as of 2011-02-28 10:43:50 → ⇥
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  Editor: jgalaz
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-This is a relatively new feature in EMAN2, and is ...
+This is a relatively new feature in EMAN2. This session will cover the beta version of a small fraction of the Single Particle Tomography (SPT) capabilities that will eventually be available.
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-The software is provided as part of EMAN2 (already installed)
+The session will cover:
1) Sub-volume extraction from tomograms using e2tomoboxer.py
2) Preparation of extracted particles for alignment
3) Reference based alignment and averaging
-Line 8:
+Line 11:
-We have prepared some sample data
+The software is provided as part of EMAN2 (already installed).
-Line 11:
+Line 14:
+We have prepared some sample data.
The tomogram in the link below comes from a tilt series of epsilon15 virion particles recorded using Zernike phase-plate technology:

[[attachment:e15phaseplate.rec]]

The following tomogram also comes from a tilt series of epsilon15 virion particles but was recorded under conventional cryoEM imaging conditions:

[[attachment:e15normal.rec]]