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| == Single Particle Tomography in EMAN2 == === Wed PM Practical === |
= Single Particle Tomography in EMAN2 = == Wednesday - P.M. == |
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| This is a relatively new feature in EMAN2, and is ... | This session will cover the new tools for single particle tomography in EMAN2. Unfortunately, this technique is very memory-intensive and compute-intensive. The 3 gb of ram available on the workshop computers is barely sufficient to do some small examples, and full 3-D alignments of large sets of particles can take many hours of computation. |
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| The software is provided as part of EMAN2 (already installed). [[attachment:spt_demo_2011.tgz]] |
So, for purposes of the tutorial, we will learn how to use the particle picker, and do a couple of small examples which can be completed in the available time. |
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| We have prepared some sample data. The tomogram in the link below comes from a tilt series of epsilon15 virion particles recorded using Zernike phase-plate technology: [[attachment:e15phaseplate.rec]] |
== DATA == [[attachment:e2spt_data.zip| e2spt_data.zip|&do=get]] |
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| The following tomogram also comes from a tilt series of epsilon15 virion particles, but was recorded under conventional cryoEM imaging conditions: [[attachment:e15normal.rec]] |
== TUTORIAL DOCUMENT == [[attachment:eman2_spt_tutorial.pdf| eman2_spt_tutorial.pdf|&do=get]] (Note that this tutorial will be passed out at the workshop, and may still change before Wed) |
Single Particle Tomography in EMAN2
Wednesday - P.M.
This session will cover the new tools for single particle tomography in EMAN2. Unfortunately, this technique is very memory-intensive and compute-intensive. The 3 gb of ram available on the workshop computers is barely sufficient to do some small examples, and full 3-D alignments of large sets of particles can take many hours of computation.
So, for purposes of the tutorial, we will learn how to use the particle picker, and do a couple of small examples which can be completed in the available time.
DATA
TUTORIAL DOCUMENT
eman2_spt_tutorial.pdf (Note that this tutorial will be passed out at the workshop, and may still change before Wed)