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| == Single Particle Tomography in EMAN2 == === Wed PM Practical === |
= Single Particle Tomography in EMAN2, 2011 WORKSHOP in Houston, TX = |
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| This is a relatively new feature in EMAN2. This session will cover the beta version of a small fraction of the Single Particle Tomography (SPT) capabilities that will eventually be available. | The SPT page has move to http://blake.bcm.edu/emanwiki/SPT/Spt |
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| The session will cover: 1) Sub-volume extraction from tomograms using e2tomoboxer.py 2) Preparation of extracted particles for alignment 3) Reference based alignment and averaging |
This is just an archaic page with the version of the tutorial used in the EMAN2 Workshop in March 2011. |
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| The software is provided as part of EMAN2 (already installed). | == OLD E2SPT USERS' GUIDE == |
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| [[attachment:spt_demo_2011.tgz]] | * Single particle tomography USER'S GUIDE (March 2011). |
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| We have prepared some sample data. The tomogram in the link below comes from a tilt series of epsilon15 virion particles recorded using Zernike phase-plate technology: |
[[attachment:e2spt_users_guide_03_2011_workshop.pdf| e2spt_users_guide_03_2011_workshop.pdf |&do=get]] |
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| [[attachment:e15phaseplate.rec]] | == DATA == Epsilon 15 virus data, used since the EMAN2 Workshop in 2010. |
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| The following tomogram also comes from a tilt series of epsilon15 virion particles but was recorded under conventional cryoEM imaging conditions: [[attachment:e15normal.rec]] |
[[http://blake.grid.bcm.edu/wikifiles/SPT/e2spt_data.zip|e2spt_data.zip]] |
Single Particle Tomography in EMAN2, 2011 WORKSHOP in Houston, TX
The SPT page has move to http://blake.bcm.edu/emanwiki/SPT/Spt
This is just an archaic page with the version of the tutorial used in the EMAN2 Workshop in March 2011.
OLD E2SPT USERS' GUIDE
* Single particle tomography USER'S GUIDE (March 2011).
e2spt_users_guide_03_2011_workshop.pdf
DATA
Epsilon 15 virus data, used since the EMAN2 Workshop in 2010.