eman2:e2tomo_more
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eman2:e2tomo_more [2025/08/05 01:05] – [Automated particle selection] steveludtke | eman2:e2tomo_more [2025/08/05 02:17] (current) – steveludtke | ||
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7. Go through all tomograms in the list and apply the network to select the particles. These particles can be viewed and modified in // | 7. Go through all tomograms in the list and apply the network to select the particles. These particles can be viewed and modified in // | ||
- | {{http:// | + | {{https:// |
Description of items on the GUI: | Description of items on the GUI: | ||
* **New/ | * **New/ | ||
- | * **!ChangeBx** : Change the box size of positive/ | + | * **ChangeBx** : Change the box size of positive/ |
* **Reference/ | * **Reference/ | ||
- | * **!TargetSize** : This controls the size of target area used for CNN training. i.e. particles should be centered in this region in positive samples, and there should not be particle features in this region in negative samples. The region is defined as a Gaussian function and value here is the sigma of the Gaussian. | + | * **TargetSize** : This controls the size of target area used for CNN training. i.e. particles should be centered in this region in positive samples, and there should not be particle features in this region in negative samples. The region is defined as a Gaussian function and value here is the sigma of the Gaussian. |
* **Learnrate** : Learning rate for the CNN training. Normally no need to change this... | * **Learnrate** : Learning rate for the CNN training. Normally no need to change this... | ||
- | * **!PtclThresh** : The intensity threshold in the neural network output to be recognized as a particle. The target of positive samples should be 1 and negative samples should be 0. | + | * **PtclThresh** : The intensity threshold in the neural network output to be recognized as a particle. The target of positive samples should be 1 and negative samples should be 0. |
- | * **!CircleSize** : The radius of circles in pixels on the tomogram slice view. This also controls the closest distance between particles. | + | * **CircleSize** : The radius of circles in pixels on the tomogram slice view. This also controls the closest distance between particles. |
* **Sum/Max** selection box : Choose between different modes of the loss function. **Sum** is used for globular particles that are generally confined in the target area. In **Max** mode, the CNN only assume there are particle features that exist within the region. It is harder to train than the **Sum** mode, but allows particles of irregular shapes, such as protein fibers. | * **Sum/Max** selection box : Choose between different modes of the loss function. **Sum** is used for globular particles that are generally confined in the target area. In **Max** mode, the CNN only assume there are particle features that exist within the region. It is harder to train than the **Sum** mode, but allows particles of irregular shapes, such as protein fibers. | ||
eman2/e2tomo_more.1754355957.txt.gz · Last modified: by steveludtke