EMAN2 is the successor to EMAN1. It is a broadly based greyscale scientific image processing suite with a primary focus on processing data from transmission electron microscopes. EMAN's original purpose was performing single particle reconstructions (3-D volumetric models from 2-D cryo-EM images) at the highest possible resolution, but the suite now also offers support for single particle cryo-ET, and tools useful in many other subdisciplines such as helical reconstruction, 2-D crystallography and whole-cell tomography. EMAN2 is capable of processing very large data sets (>100,000 particle) very efficiently (up to 20x faster than EMAN1).
The official 2.2 release is still a work in progress, but should be complete by the end of Feb 2017
EMAN is free software, supported by NIH Grants. To keep our grant funding it is critical that you cite EMAN2 when you use it in a publication in any significant way.
Primary EMAN2 paper:
EMAN2 high resolution refinement methods:
Methods for analysis of conformational and compositional variability:
Methods for subtomogram averaging:
- Live Tutorials
Past video tutorials: https://www.youtube.com/c/SteveLudtke
- User Documentation
Advanced Users & Programmers
FAQ - Please ask your questions in the Google Group, answers to common questions will be posted here as well as in the Group
- Mailing list for EMAN2 discussions:
EMAN2 is now a fully usable reconstruction package, including parallelism support. However, in case you aren't ready to abandon EMAN1 yet, EMAN1 and EMAN2 can be installed in the same user account with no conflicts. All EMAN2 programs, including GUI programs, are written in the easy-to-learn Python scripting language. This permits knowledgeable end-users to customize any of the code with unprecedented ease. If you aren't an advanced user, you can still make use of the integrated GUI and all of EMAN2's command-line programs. Any programs in EMAN2 with an EMAN1 equivalent are likely substantially improved over their EMAN1 equivalents. For example e2pdb2mrc.py is ~10x faster than the EMAN1 pdb2mrc. 3-D refinements are typically 5-25x faster than in EMAN1 (with the correct options).