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| Structural biology overview {{attachment:struct_bio.png||width=400}} | Structural biology overview |
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| Transmission electron microscopes (TEM) {{attachment:tem.png||width=400}} | {{attachment:struct_bio.png||width=400}} |
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| TEM grids {{attachment:grid.png||align="right",width=400}} | Transmission electron microscopes (TEM) |
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| Movie-mode imaging {{attachment:movie.png||align="right",width=400}} | {{attachment:tem.png||width=400}} |
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| Basic image processing {{attachment:imgproc.png||align="right",width=400}} | TEM grids |
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| Fourier transforms {{attachment:fft.png||align="right",width=400}} | {{attachment:grid.png||width=400}} |
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| Single particle analysis {{attachment:spa.png||align="right",width=400}} | Movie-mode imaging |
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| Contrast transfer function (CTF) {{attachment:ctf.png||align="right",width=400}} | {{attachment:movie.png||width=400}} |
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| Resolution measurement via Fourier Shell Correlation (FSC) {{attachment:fsc.png||align="right",width=400}} | Basic image processing |
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| Electron cryo-tomography (cryoET) {{attachment:cryoet.png||align="right",width=400}} | {{attachment:imgproc.png||width=400}} |
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| Subtomogram Averaging {{attachment:subtomoavg.png||align="right",width=400}} | Fourier transforms {{attachment:fft.png||width=400}} Single particle analysis {{attachment:spa.png||width=400}} Contrast transfer function (CTF) {{attachment:ctf.png||width=400}} Resolution measurement via Fourier Shell Correlation (FSC) {{attachment:fsc.png||width=400}} Electron cryo-tomography (cryoET) {{attachment:cryoet.png||width=400}} Subtomogram Averaging {{attachment:subtomoavg.png||width=400}} |
Michael Bell, Ludtke Lab (2014 - 2019)
All are welcome to use the materials provided here. Credit is appreciated but not required.
Thesis
Note: All protein illustrations in are licensed under a Creative Commons Attribution 4.0 International license by David S. Goodsell and the RCSB PDB.
Thesis Defense Presentation
Posters
cryoet_poster.pdf, cryoet_poster.pptx
Note, these posters are have been uploaded without modification. There may be typos.
Talks
microscopy_microanalysis_2018.ppt
Select Thesis Figures
Structural biology overview
Transmission electron microscopes (TEM)
TEM grids
Movie-mode imaging
Basic image processing
Fourier transforms
Single particle analysis
Contrast transfer function (CTF)
Resolution measurement via Fourier Shell Correlation (FSC)
Electron cryo-tomography (cryoET)
Subtomogram Averaging
