Frealign

Overview

´╗┐Frealign is a program for high-resolution refinement of 3D reconstructions from cryo-EM images of single particles, originally developed at the MRC/LMB. It is now developed and maintained by the Grigorieff lab at Brandeis.

The main Frealign documentation can be found here.

Installation

Linux:

1) Download Frealign here

2) Move the Frealign tar file to your desired installation location

3) Untar Frealign: tar -xvf frealign_v8.09_######.tar.gz

4) In your .bashrc, include the command:

5) Frealign is ready to be run. From the command line:

6) Note: the frealign_v8.exe you downloaded is a 64 bit Linux executable.

OSX: Since the Mac doesn't come with a Fortran compiler, we include a binary courtesy of Chuck Sindelar in Dr. Grigorieff's lab.

1) Download Frealign here

2) chmod a+x the frealign executable if it is not already execute permissed

3) move frealign to a folder in your path or add this to your .profile

Windows: Dr. Grigorieff has kindly made a windows executable available for download, since establishing a Fortran build environment on Windows is not for the faint of heart.

1) Download the windows executable here.

Bug fix version of e2runfrealign.py for EMAN2 workshop 2011 Download here.

Ws2011/Frealign (last edited 2011-03-16 18:31:32 by gtang)