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| === ? Practical === | === Overview === [[http://emlab.rose2.brandeis.edu/frealign|Frealign] is a program for high-resolution refinement of 3D reconstructions from cryo-EM images of single particles, originally developed at the MRC/LMB. It is now developed and maintained by the Grigorieff lab at Brandeis. |
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| Frealign is a software suite for ... | The main Frealign documentation can be [[ http://emlab.rose2.brandeis.edu/sites/default/files/readme_frealign.txt|found here]]. |
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| You can download Frealign here: [[ ??? ]]] |
=== Installation === Linux: |
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| You can download data ... [[attachment:frealign_demo_2011.tgz]] ... |
1) [[http://emlab.rose2.brandeis.edu/frealign|Download Frealign here]] |
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| etc. | 2) Move the Frealign tar file to your desired installation location . ex. mv frealign_v8.09_######.tar.gz /home/username/ 3) Untar Frealign: tar -xvf frealign_v8.09_######.tar.gz 4) In your .bashrc, include the command: . export PATH=/home/username/frealign_v8.09/bin:$PATH 5) Frealign is ready to be run. From the command line: . frealign_v8.exe OSX: Since the Mac doesn't come with a Fortran compiler, we include a binary courtesy of Chuck Sindelar in Dr. Grigorieff's lab. 1) [[attachment:frealign|Download Frealign here]] 2) chmod a+x frealign 3) move frealign to a folder in your path Windows: Dr. Grigorieff has kindly made a windows executable available for download, since establishing a Fortran build environment on Windows is not for the faint of heart. 1) [[attachment:frealign.exe|Download the windows executable here]]. |
Frealign
Overview
[[http://emlab.rose2.brandeis.edu/frealign|Frealign] is a program for high-resolution refinement of 3D reconstructions from cryo-EM images of single particles, originally developed at the MRC/LMB. It is now developed and maintained by the Grigorieff lab at Brandeis.
The main Frealign documentation can be found here.
Installation
Linux:
2) Move the Frealign tar file to your desired installation location
- ex. mv frealign_v8.09_######.tar.gz /home/username/
3) Untar Frealign: tar -xvf frealign_v8.09_######.tar.gz
4) In your .bashrc, include the command:
- export PATH=/home/username/frealign_v8.09/bin:$PATH
5) Frealign is ready to be run. From the command line:
- frealign_v8.exe
OSX: Since the Mac doesn't come with a Fortran compiler, we include a binary courtesy of Chuck Sindelar in Dr. Grigorieff's lab.
2) chmod a+x frealign
3) move frealign to a folder in your path
Windows: Dr. Grigorieff has kindly made a windows executable available for download, since establishing a Fortran build environment on Windows is not for the faint of heart.