Frealign

Overview

Frealign is a program for high-resolution refinement of 3D reconstructions from cryo-EM images of single particles, originally developed at the MRC/LMB. It is now developed and maintained by the Grigorieff lab at Brandeis.

The main Frealign documentation can be found here.

Installation

Linux:

1) Download Frealign here

2) Move the Frealign tar file to your desired installation location

• ex. mv frealign_v8.09_######.tar.gz /home/username/

3) Untar Frealign: tar -xvf frealign_v8.09_######.tar.gz

4) In your .bashrc, include the command:

• export PATH=/home/username/frealign_v8.09/bin:$PATH

5) Frealign is ready to be run. From the command line:

• frealign_v8.exe

OSX: Since the Mac doesn't come with a Fortran compiler, we include a binary courtesy of Chuck Sindelar in Dr. Grigorieff's lab.

1) Download Frealign here

2) chmod a+x frealign

3) move frealign to a folder in your path

Windows: Dr. Grigorieff has kindly made a windows executable available for download, since establishing a Fortran build environment on Windows is not for the faint of heart.

1) Download the windows executable here.