Frealign

Overview

FreAlign is a software suite for high-resolution refinement of 3D reconstructions from cryo-EM images of single particles.

You can download FreAlign here: http://emlab.rose2.brandeis.edu/frealign

And view the FreAlign documentation here: http://emlab.rose2.brandeis.edu/sites/default/files/readme_frealign.txt

Installation

Linux:

1) Download FreAlign from the above link.

2) Move the FreAlign tar file to your desired installation location

• ex. mv frealign_v8.09_######.tar.gz /home/username/

3) Unzip FreAlign: tar -xvf frealign_v8.09_######.tar.gz

4) In your .bashrc, include the command:

• export PATH=/home/username/frealign_v8.09/bin:$PATH

5) FreAlign is ready to be run. From the command line:

• frealign_v8.exe

OSX:

1) Download FreAlign from the above link.

2) Move the FreAlign tar file to your desired installation location

• ex. mv frealign_v8.09_######.tar.gz /home/username/

3) Unzip FreAlign: tar -xvf frealign_v8.09_######.tar.gz

4) Ensure you have the fortran compiler installed.

5) Change to the source directory in your frealign installation directory:

• cd /home/username/frealign_v8.09/src

6) Use the appropriate Makefile. Assuming you are using the Intel 64-bit x86:

• make -f Makefile_OSX_86-64_intel