Rosetta

Thursday PM Practical

Rosetta is an object-oriented software suite which provides a robust system for predicting and designing protein structures, protein folding mechanisms, and protein-protein interactions.

For the tutorial, you can download binaries of Rosetta for:

• Linux: rosetta_binaries_linux-mar7-11.tar.gz

• Mac: rosetta_binaries_mac_mar7_11.tar.gz

• Windows: Sorry. Not available

Please also download the database suitable for all the systems:

• rosetta_database-mar7-11.tar.gz

Afterwards, please add the following two lines in your .bashrc file and do "source ~/.bashrc". Mac users should modify the lines accordingly.

export ROSETTA3_SRC=/home/eman/eman2011/rosetta/rosetta_binaries_linux
export ROSETTA3_DB=/home/eman/eman2011/rosetta/rosetta_database

For installation of the standard release, the latest release of Rosetta, version 3.2, may be downloaded at http://www.rosettacommons.org/software. The software is free to academic users, who may get a license (and download link) by clicking on the appropriate link on the left side of the page. The user manual, containing more detailed installation and usage information, is available online at http://www.rosettacommons.org/manuals/archive/rosetta3.2_user_guide.

A brief installation guide for the standard release can be found at: Installation_Guide.pdf

Tutorial data is here:

• Linux: cryo_em_tutorial-linux-mar7-11.tar.gz

• Mac: cryo_em_tutorial-mac-mar7-11.tar.gz

Tutorial documentation is here: Rosetta_Density_Tutorial.pdf (though this will also be handed out at the workshop, and may be updated before thursday)