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| * GroEL Crystal Structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=1XCK|PDB:1XCK]] * Rotavirus structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=1QHD|PDB:1QHD]] |
* GroEL structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=1XCK|1xck.pdb]] * Mm-cpn structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=3LOS|3los.pdb]] * Ribosome structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=1VSA|1vsa.pdb]], [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=2OW8|2ow8.pdb]] * Rotavirus structure: [[http://www.pdb.org/pdb/download/downloadFile.do?fileFormat=pdb&compression=NO&structureId=1QHD|1qhd.pdb]] |
Segger
Wed AM Practical
Segger is a tool for segmentind density maps, and for quick rigid-body docking of structures into density maps [1].
The software is provided as part of Chimera which will already be installed for the workshop.
Segmentation
Data:
GroEL at 10.0Å resolution: emd_1080.map.gz
GroEL at 4.2Å resolution: emd_5001.map.gz
Mm-cpn at 4.3Å resolution: emd_5137.map.gz
Mm-cpn at 8.0Å resolution: emd_5140.map.gz
Ribosome at 6.4Å resolution: emd_5030.map.gz
Rotavirus at 3.8Å resolution: emd_1461.map.gz
Rigid-body Docking
Data:
Here is some sample data to download:segger_demo_2011.tgz
References
[1] Pintilie, G., Zhang, J., Goddard, T., Chiu, W., Gossard, D. Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions, Journal of Structural Biology 170(3), pp 427-438, 2010