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== Beginners: Introductory Tutorials == = EMAN2 TUTORIALS =
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==== 2D single particle analysis ==== == Beginners (introductory tutorials) ==
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* The old SPR tutorial - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]])

* [[Ws2011/Eman2|The NEW tutorial from the 2011 EMAN2 workshop, compatible with EMAN 2.01]]

==== 3D single particle tomography ====
* NEW single particle tomography USERS' GUIDE. [Partially used for the SPT tutorial from the 2011 EMAN2 workshop].

[[attachment:e2spt_users_guide.pdf| e2spt_users_guide.pdf|&do=get]]
''Please note that we are continuously developing and updating the tutorials. Items that are greyed out
are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial
please feel free to email sludtke@bcm.edu.''
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==== 2D assessment of heterogeneity ====
* [[attachment:population_dynamics_2009_11.pdf|Full tutorial on 2-D analysis for assessment of structural heterogeneity and population dynamics]]
=== Video tutorials and discussions ===
  * [[EMAN2/VideoTutorials|EMAN2 Video Tutorials]]
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== Advanced Users: Using EMAN2 through a Python prompt (e2.py) == === Written Tutorials ===

==== Single particle analysis (SPA) - 2D particles ====
 * 2016 Summer Tutorial for EMAN2.12 June 2016 snapshots
  * [[attachment:EMAN2-13_pre_Tutorial.pdf||&do=get]]
   * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]]
  * [[attachment:EMAN2-13_pre_Heterogeneity.pdf||&do=get]]
  * see also: http://www.sciencedirect.com/science/article/pii/S0076687916300362
 * 2015 Fall Tutorial for EMAN2.12
  * [[attachment:EMAN2-12_Tutorial.pdf||&do=get]]
  * Required data - [[attachment:Bgal.zip||&do=get]]
  * [[attachment:EMAN2-12_Tutorial2_heterogeneity.pdf||&do=get]]
  * Required data - [[attachment:ribosome.zip||&do=get]]
 * [[Ws2015|2015 Workshop Tutorials and Demo data]] - This includes updated tutorials on both high resolution single particle analysis and 2D/3D heterogeneity analysis along with sample data, freshly updated for the EMAN2.12 release. The workshop also included a number of other related topics also available on that page.
 * [[EMAN2/Eman1Transition|EMAN1 to EMAN2 Transition Guide]] - Also with useful quickstart guides for users of other software
 * [[OxfordWs2012|Tilt Validation tutorial]]
 * [[RctTutorial|Random Conical Tilt Tutorial]]
 * [[EMAN2/Programs/convnet_pickparticle|An experimental new particle picker]] (requires installation of additional libraries)

==== Single particle tomography (SPT) - 3D particles ====
BOTH the tutorial in .pdf format and the data needed for the tutorial in a .zip file are provided in the page linked below:

 * [[SPT/Spt|Single particle tomography]]

==== Automated Cellular Tomogram Annotation ====
 * [[Programs/tomoseg|Tomogram Annotation]]

==== Using the Built-In Modules from Other Software ====
 * [[EMAN2/Programs/e2refinetorelion2d/e2refinetorelion2dtutorial|2D Class Averaging with Relion]]
 * [[EMAN2/Programs/e2refinetorelion3d/e2refinetorelion3dtutorial|3D Refinement with Relion]]
 * [[EMAN2/Programs/e2refinetofrealign/e2refinetofrealigntutorial|3D Refinement with FreAlign]]

=== Out of date tutorials (provided for data access, etc.) ===
 * [[Ws2014|Tutorial from the 2014 Workshops in Shanghai, Brisbane and online]] - Based on EMAN2.1 beta4. Full single particle reconstruction tutorial
 * Single particle reconstruction tutorial from 2008 Workshop ( - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]])
 * [[Ws2011/Eman2|Tutorial from the 2011 EMAN2 workshop (Data on same page)]] - Based on EMAN2.01. Full single particle reconstruction tutorial
 * [[Ws2012/EMAN2|Tutorial from the 2012 Workshop in Singapore (Data on same page)]] - Based on EMAN2.06. Full single particle reconstruction tutorial
 * [[Ws2013/EMAN2|Tutorial from the 2013 Workshop in Beijing (Data on same page)]] - Based on EMAN2.1alpha. Full single particle reconstruction tutorial


== Advanced (EMAN2 through the ''e2.py'' Python prompt) ==

EMAN2 TUTORIALS

Beginners (introductory tutorials)

Please note that we are continuously developing and updating the tutorials. Items that are greyed out are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial please feel free to email sludtke@bcm.edu.

Video tutorials and discussions

Written Tutorials

Single particle analysis (SPA) - 2D particles

Single particle tomography (SPT) - 3D particles

BOTH the tutorial in .pdf format and the data needed for the tutorial in a .zip file are provided in the page linked below:

Automated Cellular Tomogram Annotation

Using the Built-In Modules from Other Software

Out of date tutorials (provided for data access, etc.)

Advanced (EMAN2 through the ''e2.py'' Python prompt)

* Generate and display an EMAN2 test image

* Rotate, translate, scale or mirror an image

* Add and multiply images, add and multiply by constants

* Convert a real/imaginary image into an amplitude/phase image and vice versa

* Iterate through the pixels of an image

* Make a projection of a 3D model, project through the asymmetric unit

2d_image_3dplot_2.png

* Change log level in EMAN2

EMAN2/Tutorials (last edited 2024-04-05 01:51:27 by MuyuanChen)