Differences between revisions 70 and 136 (spanning 66 versions)
Revision 70 as of 2012-05-04 12:14:20
Size: 2278
Editor: SteveLudtke
Comment:
Revision 136 as of 2018-08-25 01:03:18
Size: 4859
Editor: SteveLudtke
Comment:
Deletions are marked like this. Additions are marked like this.
Line 6: Line 6:
are planned but the tutorials have not yet been completed. If you have a request for a particular tutorial are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial
Line 10: Line 10:
=== Video tutorials and discussions ===
  * [[EMAN2/VideoTutorials|EMAN2 Video Tutorials]]

=== Written Tutorials ===

==== Short tutorials and info sheets ====
 * [[EMAN2/NegativeStain]] - special issues that impact processing negative stain single particle images
 * [[attachment:image_info.pdf|Information about images]]
 * [[attachment:image_proc.pdf|Filtering and basic image processing]]
 * [[attachment:ctf_sim.pdf|CTF Simulator]]
Line 11: Line 22:
 * 2018 May Tutorial for EMAN2.21a
  * [[attachment:EMAN2-21Tutorial.pdf||&do=get]] (version 1)
   * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]]
 * 2017 February Tutorial for EMAN2.2
  * [[attachment:EMAN2-2Tutorial.pdf||&do=get]]
   * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]]
 * 2016 Summer Tutorial for EMAN2.12 June 2016 snapshots
  * [[attachment:EMAN2-13_pre_Tutorial.pdf||&do=get]]
   * [[http://blake.bcm.edu/dl/workshop_2016_bgal.zip|Tutorial Data Download]]
  * [[attachment:EMAN2-13_pre_Heterogeneity.pdf||&do=get]]
  * see also: http://www.sciencedirect.com/science/article/pii/S0076687916300362
 * 2015 Fall Tutorial for EMAN2.12
  * [[attachment:EMAN2-12_Tutorial.pdf||&do=get]]
  * Required data - [[attachment:Bgal.zip||&do=get]]
  * [[attachment:EMAN2-12_Tutorial2_heterogeneity.pdf||&do=get]]
  * Required data - [[attachment:ribosome.zip||&do=get]]
 * [[Ws2015|2015 Workshop Tutorials and Demo data]] - This includes updated tutorials on both high resolution single particle analysis and 2D/3D heterogeneity analysis along with sample data, freshly updated for the EMAN2.12 release. The workshop also included a number of other related topics also available on that page.
Line 12: Line 40:
 * [[EMAN2/Eman1Transition|EMAN1 to EMAN2 Transition Guide]]
 * The old SPR tutorial (2008 Workshop) - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]])
 * [[Ws2011/Eman2|The NEW tutorial from the 2011 EMAN2 workshop, compatible with EMAN 2.01 (Data on same page)]]
 * [[OxfordWs2012|The Validation tutorial from the Oxford 2012 EMAN2 workshop, compatible with EMAN Nightly Build (Data on same page)]]
 * [[attachment:rct_tutorial.pdf|Tutorial on using EMAN2 for Random Conical Tilt reconstructions|&do=get]]
 * [[EMAN2/Programs/convnet_pickparticle|tutorial for the Neural network particle picker]] (requires CUDA for good performance)
 * [[EMAN2/Eman1Transition|EMAN1 to EMAN2 Transition Guide]] - Also with useful quickstart guides for users of other software
 * [[OxfordWs2012|Tilt Validation tutorial]]
 * [[RctTutorial|Random Conical Tilt Tutorial]]
Line 18: Line 45:
==== Complete workflow for cellular tomography and subtomogram averaging ====
 * [[e2tomo|EMAN2 tomography workflow tutorial]]
Line 19: Line 48:
==== Single particle tomography (SPT) - 3D particles ====
 * NEW single particle tomography USERS' GUIDE.
 [[attachment:e2spt_users_guide.pdf| e2spt_users_guide.pdf|&do=get]]
==== Automated Cellular Tomogram Annotation using Neural Networks ====
 * [[EMAN2/Programs/tomoseg|Tomogram Annotation]]
Line 23: Line 51:
==== 2D assessment of heterogeneity ====
 * [[attachment:population_dynamics_2009_11.pdf|Full tutorial on 2-D analysis for assessment of structural heterogeneity and population dynamics]] (under construction)
==== Using the Built-In Modules from Other Software ====
 * [[EMAN2/Programs/e2refinetorelion2d/e2refinetorelion2dtutorial|2D Class Averaging with Relion]]
 * [[EMAN2/Programs/e2refinetorelion3d/e2refinetorelion3dtutorial|3D Refinement with Relion]]
 * [[EMAN2/Programs/e2refinetofrealign/e2refinetofrealigntutorial|3D Refinement with FreAlign]]
Line 26: Line 56:
==== Adding an e2program to the program manger workflow ====
 * [[EMAN2PMWorkflow|Modifying the EMAN2 projectmanager workflow]]
=== Out of date tutorials (provided for data access, etc.) ===
 * [[SPT/Spt|Single particle tomography]] - Tutorial for deprecated Single particle tomography (SPT) workflow.
 * [[Ws2014|Tutorial from the 2014 Workshops in Shanghai, Brisbane and online]] - Based on EMAN2.1 beta4. Full single particle reconstruction tutorial
 * Single particle reconstruction tutorial from 2008 Workshop ( - [[attachment:workflow_tutorial_2010_08_25.pdf|Full tutorial on single particle reconstruction using the workflow]] (uses [[http://blake.grid.bcm.edu/dl/data/workshop5.iso|Workshop demo data DVD]])
 * [[Ws2011/Eman2|Tutorial from the 2011 EMAN2 workshop (Data on same page)]] - Based on EMAN2.01. Full single particle reconstruction tutorial
 * [[Ws2012/EMAN2|Tutorial from the 2012 Workshop in Singapore (Data on same page)]] - Based on EMAN2.06. Full single particle reconstruction tutorial
 * [[Ws2013/EMAN2|Tutorial from the 2013 Workshop in Beijing (Data on same page)]] - Based on EMAN2.1alpha. Full single particle reconstruction tutorial
Line 30: Line 65:

EMAN2 TUTORIALS

Beginners (introductory tutorials)

Please note that we are continuously developing and updating the tutorials. Items that are greyed out are planned but the tutorials have not yet been completed. Most tutorials include the necessary data. If you have a request for a particular tutorial please feel free to email sludtke@bcm.edu.

Video tutorials and discussions

Written Tutorials

Short tutorials and info sheets

Single particle analysis (SPA) - 2D particles

Complete workflow for cellular tomography and subtomogram averaging

Automated Cellular Tomogram Annotation using Neural Networks

Using the Built-In Modules from Other Software

Out of date tutorials (provided for data access, etc.)

Advanced (EMAN2 through the ''e2.py'' Python prompt)

* Generate and display an EMAN2 test image

* Rotate, translate, scale or mirror an image

* Add and multiply images, add and multiply by constants

* Convert a real/imaginary image into an amplitude/phase image and vice versa

* Iterate through the pixels of an image

* Make a projection of a 3D model, project through the asymmetric unit

2d_image_3dplot_2.png

* Change log level in EMAN2

EMAN2/Tutorials (last edited 2024-04-05 01:51:27 by MuyuanChen)