Material for NCMI 2011 Workshop on CryoEM Single Particle Analysis

This page contains links to the software and data you need to download for each of the specific tutorials at the workshop. People using the on-site desktop PCs do not need to install anything in advance, unless they wish to do something on their laptops as well.

Pleae note: You will need to have everything installed and working on your laptop before the workshop. We are happy to help you with installation/setup problems via email before the workshop, but we cannot individually help 90+ laptop users AT the workshop with installation problems. If you do not wish to participate in one or more of the tutorials below, you can, of course, skip that section.

Required software/data:

EMAN2

General purpose image processing and refinement

Used throughout the workshop.

Chimera

Visualization/Interactive post procesing

Used throughout the workshop.

Gorgon

Model building, secondary structure detection

Tutorial on day 4

Rosetta

High resolution model building

Used for mini-tutorial on day 4

DireX

Dynamic modeling

Demo on day 4

EMEN2

Database/Electronic Notebook

Demo/tutorial on Day 4

Frealign

Single particle refinement

EMAN2/Frealign tutorial on day 3

Data only:

SPARX

demo data for day 2

Segger

Interactive segmentation

Brief demo/tutorial Day 3

Single particle tomography

EMAN2 used for processing. Tutorial day 3